Development of a reverse transcription-quantitative PCR assay for detection of salivirus/klassevirus.

نویسندگان

  • Eiji Haramoto
  • Masaaki Kitajima
  • Mikie Otagiri
چکیده

A broadly reactive and highly sensitive reverse transcription-quantitative PCR assay to detect salivirus/klassevirus was developed. By means of the developed assay, salivirus/klassevirus was detected in 13 (93%) raw sewage, 4 (29%) secondary-treated sewage, and 9 (16%) river water samples, with a maximum concentration of 9.7 × 10(6) copies/liter.

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عنوان ژورنال:
  • Applied and environmental microbiology

دوره 79 11  شماره 

صفحات  -

تاریخ انتشار 2013